Practical example: from raw gas concentration data to clean ecosystem gas fluxes.

In this example we will process a dataset from the Plant Functional Traits Course 6 (PFTC6; Norway, 2022). Net ecosystem exchange (NEE), ecosystem respiration (ER), air and soil temperature and photosynthetically active radiation (PAR) were recorded over the course of 24 hours at an experimental site called Liahovden, situated in an alpine grassland in southwestern Norway. Those data are available in the Fluxible R package. To work with your own data, you need to import them as a dataframe object in your R session. For import examples, please see vignette("data-prep", package = "fluxible").

Input

The CO2 concentration data as well as air and soil temperature and PAR were recorded in a dataframe named co2_liahovden. The metadata for each measurement are in a dataframe named record_liahovden. This dataframe contains the starting time of each measurement, the measurement type (NEE or ER) and the unique plot ID (called turfs in this experiment).

Structure of the CO2 concentration data (co2_liahovden):

#> tibble [89,692 × 5] (S3: tbl_df/tbl/data.frame)
#>  $ datetime : POSIXct[1:89692], format: "2022-07-27 05:34:49" ...
#>  $ temp_air : num [1:89692] 3 NA NA NA NA NA NA NA NA NA ...
#>  $ temp_soil: num [1:89692] 2.96 NA NA NA NA NA NA NA NA NA ...
#>  $ conc     : num [1:89692] 468 469 468 468 468 ...
#>  $ PAR      : num [1:89692] 2.59 NA NA NA NA NA NA NA NA NA ...

Structure of the meta data (record_liahovden):

#> tibble [138 × 4] (S3: tbl_df/tbl/data.frame)
#>  $ turfID: chr [1:138] "4 AN1C 4" "4 AN1C 4" "27 AN3C 27" "27 AN3C "..
#>  $ type  : chr [1:138] "NEE" "ER" "NEE" "ER" ...
#>  $ round : num [1:138] 1 1 1 1 1 1 2 2 2 2 ...
#>  $ start : POSIXct[1:138], format: "2022-07-27 05:37:30" ...

Attributing meta-data

We use the flux_match function to slice each measurement in the CO2 concentration data and discard the recordings in between. The two inputs are raw_conc, the dataframe containing field measured raw gas concentration, and field_record, the meta data dataframe with the start of each measurement. Then f_datetime is the column containing date and time in the gas concentration dataframe, and start_col the column containing the start date and time of each measurement in the meta data dataframe. The length of the measurements is provided with measurement_length (in seconds). Alternatively, a column indicating the end time and date of each measurement can be provided to end_col, with fixed_length = FALSE. The time_diff argument allow to account for a consistant time difference (in seconds) between the two inputs. This value is added to the datetime column of the gas concentration dataset.

library(fluxible)

conc_liahovden <- flux_match(
  raw_conc = co2_liahovden, # dataframe with raw gas concentration
  field_record = record_liahovden, # dataframe with meta data
  f_datetime = datetime, # column containing date and time
  start_col = start, # start date and time of each measurement
  measurement_length = 220, # length of measurements (in seconds)
  fixed_length = TRUE, # the length of the measurement is a constant
  time_diff = 0 # time difference between f_datetime and start_col
)

Model fitting

We fit a model and obtain the slope at \(t_0\), which is needed for the flux calculation, with the flux_fitting function. In this example we use the exp_zhao18 model (Zhao et al., 2018). The exp_zhao18 is a mix of an exponential and linear model - thus fitting all fluxes independantly from curvature - and includes \(t_0\) as a fitting parameter (its implementation is described in vignette("zhao18", package = "fluxible")). A similar model but with the option to manually set \(t_0\) is exp_tz. Other available models are: linear for a linear fit, quadratic for a quadratic fit, and exp_hm for the original HM model (Hutchinson and Mosier, 1981).

The conc_df argument is the dataframe with gas concentration, date and time, and start and end of each measurement, ideally produced with flux_match (see vignette("data-prep", package = "fluxible") for requirements to bypass flux_match). Then f_conc and f_datetime are, similarly as in flux_match, the gas concentration and corresponding datetime column. The arguments f_start, f_end, and f_fluxid are produced by flux_match. They indicate, respectively, the start, end and unique ID of each measurement. The model chosen to fit the gas concentration is provided with fit_type. The user can decide to restrict the focus window before fitting the model with the start_cut and end_cut arguments. For the models quadratic, exp_tz, and exp_hm, t_zero needs to be provided to indicate how many seconds after the start of the focus window should the slope be calculated. Arguments cz_window, b_window, a_window and roll_width are specific to the automatic fitting of the exp_zhao18 and exp_tz models and are described in vignette("zhao18", package = "fluxible"). We recommend keeping the default values.

slopes_liahovden <- flux_fitting(
  conc_df = conc_liahovden, # the output of flux_match
  f_conc = conc, # gas concentration column
  f_datetime = datetime, # date and time column
  f_start = f_start, # start of each measurement, provided by flux_match
  f_end = f_end, # end of each measurement, provided by flux_match
  f_fluxid = f_fluxid, # unique ID for each measurement, provided by flux_match
  fit_type = "exp_zhao18", # the model to fit to the gas concentration
  start_cut = 0, # seconds to prune at the start before fitting
  end_cut = 0 # seconds to prune at the end of all measurements before fitting
)

Quality checks and visualizations

The function flux_quality is used to provide diagnostics about the quality of the fit, potentially advising to discard some measurements or replace them by zero. The main principle is that the user sets thresholds on diagnostics (depending on the model used) to flag the measurements according to the quality of the data and the model fit. Those quality flags are then used to provide f_slope_corr, a column containing the advised slope to use for calculation. The force_ arguments allow the user to override this automatic flagging by providing a vector of fluxIDs. The ambient_conc and error arguments are used to detect measurements starting outside of a reasonable range (the mean of the three first gas concentration data points is used, independantly from the focus window). The minimal detectable slope is calculated as \(\frac{2 \times \text{instr error}}{\text{length of measurement}}\) and is used to detect slopes that should be replaced by zero. Other arguments are described in the function documentation (displayed with ?flux_quality). The function flux_flag_count provides a table with the counts of quality flags, which is convenient for reporting on the dataset quality, and can also be done on the final flux dataset. This table is also printed as a side effect of flux_quality.

flags_liahovden <- flux_quality(
  slopes_df = slopes_liahovden,
  f_conc = conc,
  # force_discard = c(),
  # force_ok = c(),
  # force_zero = c(),
  # force_lm = c(),
  # force_exp = c(),
  ambient_conc = 421,
  error = 100,
  instr_error = 5
)
#> 
#>  Total number of measurements: 138
#> 
#>  ok   109     79 %
#>  zero     27      20 %
#>  discard      2   1 %
#>  force_discard    0   0 %
#>  start_error      0   0 %
#>  no_data      0   0 %
#>  force_ok     0   0 %
#>  force_zero   0   0 %
#>  force_lm     0   0 %
#>  no_slope     0   0 %

flux_flag_count(flags_liahovden)
#> # A tibble: 10 × 3
#>    f_quality_flag     n  ratio
#>    <fct>          <int>  <dbl>
#>  1 ok               109 0.790 
#>  2 zero              27 0.196 
#>  3 discard            2 0.0145
#>  4 force_discard      0 0     
#>  5 start_error        0 0     
#>  6 no_data            0 0     
#>  7 force_ok           0 0     
#>  8 force_zero         0 0     
#>  9 force_lm           0 0     
#> 10 no_slope           0 0

The function flux_plot provides plots for a visual assessment of the measurements, explicitly displaying the quality flags from flux_quality and the cuts from flux_fitting. Note that different values than the default can be provided to scale_x_datetime and facet_wrap by providing lists of arguments to scale_x_datetime_args and facet_wrap_args respectively.

flags_liahovden |>
  # we show only a sample of the plots in this example
  dplyr::filter(f_fluxid %in% c(54, 95, 100, 101)) |>
  flux_plot(
    f_conc = conc,
    f_datetime = datetime,
    f_ylim_upper = 600, # upper limit of y-axis
    f_ylim_lower = 350, # lower limit of x-axis
    y_text_position = 450, # position of text with flags and diagnostics
    facet_wrap_args = list( # facet_wrap arguments, if different than default
      nrow = 2,
      ncol = 2,
      scales = "free"
    )
  )
Output of flux_plot for fluxid 54, 95, 100 and 101.
Output of flux_plot for fluxid 54, 95, 100 and 101.

To export the plots as pdf without printing them in one’s R session, which we recommend for large datasets, the code looks like this:

flux_plot(
  slopes_df = flags_liahovden,
  f_conc = conc,
  f_datetime = datetime,
  print_plot = FALSE, # not printing the plots in the R session
  output = "pdfpages", # the type of output
  f_plotname = "plots_liahovden" # filename for the pdf file
)

If the argument f_plotname is left empty (the default), the name of the slopes_df object will be used (flags_liahovden in our case). The pdf file will be saved in a folder named f_quality_plots.

Based on the quality flags and the plots, the user can decide to run flux_fitting and/or flux_quality again with different parameters. Here we will cut the last 60 seconds of the fluxes (cutting the last third). We also detected a flux that do not look correct. Sometimes measurements will pass the automated quality control but the user might have reasons to still discard them (or the opposite). That is what the force_discard, force_ok, force_lm and force_zero arguments are for. In our example, for the measurement with fluxID 101, the exponential model is not providing a good fit (resulting in the flux being discarded) due to some noise at the start of the measurement. We take the decision to force the use of the linear model instead, because it seems to fit much better (given that the flux looks quite flat, we could also force it to be zero). This is achieved with force_lm = 101. Several fluxIDs can be provided to the force_ arguments by providing a vector: force_zero = c(100, 101).

The function flux_fitting is run again, with an end cut of 60 seconds:

fits_liahovden_60 <- conc_liahovden |>
  flux_fitting(
    conc,
    datetime,
    fit_type = "exp_zhao18",
    end_cut = 60 # we decided to cut the last 60 seconds of the measurements
  )

Then flux_quality again, forcing the use of the linear model for fluxID 101:

flags_liahovden_60 <- fits_liahovden_60 |>
  flux_quality(
    conc,
    force_lm = 101 # we force the use of the linear model for fluxid 101
  )
#> 
#>  Total number of measurements: 138
#> 
#>  ok   127     92 %
#>  zero     8   6 %
#>  discard      2   1 %
#>  force_lm     1   1 %
#>  force_discard    0   0 %
#>  start_error      0   0 %
#>  no_data      0   0 %
#>  force_ok     0   0 %
#>  force_zero   0   0 %
#>  no_slope     0   0 %

And finally flux_plot again to check the output:

flags_liahovden_60 |>
  dplyr::filter(f_fluxid %in% c(54, 95, 100, 101)) |>
  flux_plot(
    conc,
    datetime,
    f_ylim_upper = 600,
    f_ylim_lower = 350,
    y_text_position = 450,
    facet_wrap_args = list(
      nrow = 2,
      ncol = 2,
      scales = "free"
    )
  )
Output of flux_plot for fluxid 54, 95, 100 and 101 after refitting with a 60 seconds end cut.
Output of flux_plot for fluxid 54, 95, 100 and 101 after refitting with a 60 seconds end cut.

Flux calculation

Now that we are satisfied with the fit, we can calculate fluxes with flux_calc, which applies the following equation:

\[ \text{flux}=C'(t_0)\times \frac{P\times V}{R\times T\times A} \]

where

flux: the flux of gas at the surface of the plot (mmol m-2 h-1)

slope: slope estimate (ppm s-1)

P: pressure (atm)

V: volume of the chamber and tubing (L)

R: gas constant (0.082057 L atm K-1 mol-1)

T: chamber air temperature (K)

A: area of chamber frame base (m2)

The calculation is using the slope, which can either be f_slope (provided by flux_fitting and not quality checked) or f_slope_corr which is the recommended slope after quality check with flux_quality. Here the volume is defined as a constant for all the measurements but it is also possible to provide the volume as a separate column (setup_volume). The cols_ave arguments indicates which column(s), i.e. the environmental data, should be averaged for each flux. When setting the argument cut = TRUE (default), the same cut that was applied in flux_fitting will be used. The cols_sum and cols_med do the same for sum and median respectively. In the output, those columns get appended with the suffixes _ave, _sum and _med respectively. Here we recorded PAR and soil temperature in the same dataset and would like their average for each measurement. The cols_keep arguments indicates which columns should be kept. As flux_calc transforms the dataframe from one row per datapoint of gas concentration to one row per flux, the values in the columns specified in cols_keep have to be constant within each measurement (if not, rows will be repeated to accomodate for non constant values). Other columns can be nested in a column called nested_variables with cols_nest (cols_nest = "all" will nest all the columns present in the dataset, except those provided to cols_keep).

The units of gas concentration, conc_unit, can be ppm or ppb. The units of the calculated flux can be \(mmol/m^2/h\) (flux_unit = "mmol") or \(\mu mol/m^2/h\) (flux_unit = "micromol"). Temperature in the input can be in Celsius, Kelvin or Fahrenheit, and will be returned in the same unit in the output.

fluxes_liahovden_60 <- flux_calc(
  slopes_df = flags_liahovden_60,
  slope_col = f_slope_corr, # we use the slopes provided by flux_quality
  f_datetime = datetime,
  temp_air_col = temp_air,
  conc_unit = "ppm", # unit of gas concentration
  flux_unit = "mmol", # unit of flux
  temp_air_unit = "celsius",
  setup_volume = 24.575, # in liters, can also be a variable
  atm_pressure = 1, # in atm, can also be a variable
  plot_area = 0.0625, # in m2, can also be a variable
  cols_keep = c("turfID", "type"),
  cols_ave = c("temp_soil", "PAR")
)
#> tibble [138 × 10] (S3: tbl_df/tbl/data.frame)
#>  $ f_fluxid      : Factor w/ 138 levels "1","2","3","4",..: 1 2 3 4 ..
#>  $ temp_soil_ave : num [1:138] 6.96 7.01 6.83 6.83 2.5 ...
#>  $ PAR_ave       : num [1:138] 24.242 1.05 28.809 0.403 48.062 ...
#>  $ turfID        : chr [1:138] "4 AN1C 4" "4 AN1C 4" "27 AN3C 27" ""..
#>  $ type          : chr [1:138] "NEE" "ER" "NEE" "ER" ...
#>  $ f_slope_corr  : num [1:138] -0.2258 0.0718 -0.3718 0.2433 -0.2865..
#>  $ f_temp_air_ave: num [1:138] 3.21 3.3 3.15 2.96 2.81 ...
#>  $ datetime      : POSIXct[1:138], format: "2022-07-27 05:37:30" ...
#>  $ f_flux        : num [1:138] -14.09 4.48 -23.22 15.2 -17.91 ...
#>  $ f_model       : chr [1:138] "exp_zhao18" "exp_zhao18" "exp_zhao1"..

Gross Primary Production calculation

CO2 flux chambers and tents can be used to measure net ecosystem exchange (NEE) and ecosystem respiration (ER) if the user manipulates the light levels in the chamber. The difference between the two is the gross primary production (GPP), which cannot be measured isolated from ER but is often a variable of interest. The function flux_gep calculates GPP as \(GPP = NEE - ER\) and returns a dataset in long format, with NEE, ER and GPP as flux type and filling any variables specified by the user (cols_keep argument) with their values corresponding to the NEE measurement. Other type of flux than ER and NEE, if present in the dataset (e.g. light response curves, soil respiration) are kept. Each NEE and ER measurements need to be paired together for this calculation. The id_cols argument specifies which columns should be used for pairing (e.g., date, campaign). Since those measurements were done continuously for 24 hours, we added a pairID column, which is pairing each NEE measurement with its following ER measurement. This pairs the NEE and ER measurements of the same plot and same round of measurement because we systematically measured ER after NEE for each plot.

library(tidyverse)

fluxes_liahovden_60 <- fluxes_liahovden_60 |>
  mutate(
    f_fluxid = as.integer(f_fluxid),
    pairID = case_when(
      type == "NEE" ~ f_fluxid,
      type == "ER" ~ f_fluxid - 1
    ),
    f_fluxid = as_factor(f_fluxid),
    pairID = as_factor(pairID)
  )

gpp_liahovden_60 <- flux_gpp(
  fluxes_df = fluxes_liahovden_60,
  type_col = type, # the column specifying the type of measurement
  f_datetime = datetime,
  id_cols = c("pairID", "turfID"),
  cols_keep = c("temp_soil_ave", "PAR_ave"), # or "none" (default) or "all"
  nee_arg = "NEE", # default value
  er_arg = "ER" # default value
)

Structure of the flux dataset including GPP:

#> tibble [207 × 7] (S3: tbl_df/tbl/data.frame)
#>  $ datetime     : POSIXct[1:207], format: "2022-07-27 05:37:30" ...
#>  $ type         : chr [1:207] "GPP" "NEE" "ER" "GPP" ...
#>  $ f_flux       : num [1:207] -18.57 -14.09 4.48 -38.42 -23.22 ...
#>  $ temp_soil_ave: num [1:207] 6.96 6.96 7.01 6.83 6.83 ...
#>  $ PAR_ave      : num [1:207] 24.24 24.24 1.05 28.81 28.81 ...
#>  $ pairID       : Factor w/ 69 levels "1","3","5","7",..: 1 1 1 2 2 ..
#>  $ turfID       : chr [1:207] "4 AN1C 4" "4 AN1C 4" "4 AN1C 4" "27 "..

References

Hutchinson, G.L. and Mosier, A.R. (1981), Improved Soil Cover Method for Field Measurement of Nitrous Oxide Fluxes, Soil Science Society of America Journal, Vol. 45 No. 2, pp. 311–316.
Zhao, P., Hammerle, A., Zeeman, M. and Wohlfahrt, G. (2018), On the calculation of daytime CO2 fluxes measured by automated closed transparent chambers, Agricultural and Forest Meteorology, Vol. 263, pp. 267–275.